Analysis of Lycopene Content and Antioxidant Activity Test of Katokkon Chili Extract (Capsicum chinense Jacq)
DOI:
https://doi.org/10.22487/j24775185.2021.v10.i4.pp224-229Keywords:
Lycopene, antioxidants, chili katokkon, maseration, UV-Vis spectrophotometer, DPPHAbstract
This study aimed to determine lycopene contents and the antioxidant activity of katokkon chili extract. The extraction process of katokkon chili used the maceration method with the mixture of n-hexane : acetone : ethanol (2:1:1). The concentrated extract of katokkon chili was obtained through the evaporator process at a temperature of 40 ˚C. The analysis process used a UV-Vis spectrophotometer in which the absorbance reading was at λ = 472 nm. The average content of lycopene in katokkon chili extract was 1.457 mg/100g. Testing the antioxidant activity of katokkon chili extract used the DPPH (2,2-diphenyl-1-picrylhydrazyl) method with positive control the vitamin C. The absorbance readings for the antioxidant activity test were at λ = 517 nm. The test results showed that the antioxidant power of katokkon and vitamin C extracts in reducing DPPH free radicals had a similar result which was equally strong. The IC50 value for chili katokkon extract was 13.84 mg/L, and the IC50 value for vitamin C was 5.78 mg/L, so it can be concluded that chili katokkon can be an alternative source of natural antioxidants to replace vitamin C.
References
Alda, L. M., Gogoasa, I., Bordean, D. M., Gergen, I., Alda, S., Moldovan, C., & Nita, L. (2009). Lycopene content of tomatoes and tomato products. Journal of Agroalimentary Processes and Technologies, 15(4), 540–542.
Barba, A. I. O., Hurtado, M. C., Mata, M. C. S., Ruiz, V. F., & de Tejada, M. L. S. (2006). Application of a UV–vis detection-HPLC method for a rapid determination of lycopene and b -carotene in vegetables. Food Chemistry, 95(2), 328–336.
Desmiaty, Y., Alatas, F., & Sugianti, I. (2008). Pembuatan crude likopen dari buah tomat (Solanum lycopersicum) dan penentuan daya antioksidannya. Prosiding Kongres Ilmiah XVI ISFI 2008 Transformasi Ilmu dan Teknologi Dalam Praktek Profesi Kefarmasian (pp 1-3) Jakarta: Ikatan Sarjana Farmasi Indonesia.
Erawati. (2012). Uji Aktivitas antioksidan ekstrak daun garciniadaedalanthera pierre dengan metode DPPH (1,1-difenil pikrilhidrazil) dan identifikasi golongan senyawa kimia dari fraksi paling aktif. Skripsi Tidak Diterbitkan. Jakarta: Universitas Indonesia.
Flowrenzhy, D., & Harijati, N. (2017). Pertumbuhan dan produktivitas tanaman cabai katokkon (Capsicum chinense Jacq) di ketinggian 600 meter dan 1200 meter di atas permukaan laut. Biotropika: Journal of Tropical Biology, 5(2), 44–53.
Hastono, S. P., & Sabri, L. (2011). Statistic kesehatan. Jakarta: Rajawali Pers.
Juniarti, Osmeli, D., & Yuhernita. (2009). Kandungan senyawa kimia, uji toksisitas (Brine shrimp lethality test) dan antioksidan (1,1-diphenyl-2-pikrilhydrazyl) dari ekstrak daun saga (Abrus precatorius L.). Makara Sains, 13(1), 50–54.
Khopkar, S. M. (2003). Konsep dasar kimia analitik. Jakarta: UI-Press.
Masrifah, Rahman, N., & Abram, P. H. (2017). Uji aktivitas antioksidan ekstrak daun dan kulit labu air (Lagenaria siceraria (Molina) Standl.). Jurnal Akademika Kimia, 6(2), 98–106.
Molyneux, P. (2004). The use of the stable free radical diphenylpicryl-hydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology, 26(2), 211–219.
Mutmainnah & Masluki. (2017). Pengaruh pemberian jenis pupuk organik dan anorganik terhadap pertumbuhan dan produksi cabe besar katokkon varietas lokal toraja. PERBAL: Jurnal Pertanian Berkelanjutan, 5(3), 21–30.
Nur, K., Diah, A. W. M., & Nuryanti, S. (2016). Uji aktivitas ekstrak daun palado (Agave angustifolia) sebagai antioksidan. Jurnal Akademika Kimia, 5(2), 73–78.
Octaviani, T., Guntarti, A., & Susanti, H. (2014). Penetapan kadar ß-karoten pada beberapa jenis cabe (Genus capsicum) dengan metode spektrofotometri tampak. Pharmaciana, 4(2), 101–109.
Ortega, M. H., Moreno, A. O., Navarro, M. D. H., Cevallos, G. C., Alvarez, L. D., & Mondragón, H. N. (2012). Antioxidant, antinociceptive, and anti-inflammatory effects of carotenoids extracted from dried pepper (Capsicum annuum L.). Journal of Biomedicine and Biotechnology, 2012(August), 1–10.
Prajnata, F. (2007). Agribisnis cabai hibrida. Jakarta: Penebar Swadaya.
Pratiwi, D. R., Bintang, M., & Simajuntak, P. (2014). Lelutung tokak (Tabernaemontana macrocarpa Jack.) as source of bioactive substances, antioxidant and anticancer. Jurnal Ilmu Kefarmasian Indonesia, 12(2), 267–272.
Puspita, D., Tjahjono, J. D., Samalukang, Y., Toy, B. A. I., & Totoda, N. W. (2018). Isolation and test of pigment thermostability of katokon chili (Capsicum chinense Jacq). Journal of Food and Life Science, 2(1), 9–16.
Rao, A. V, & Rao, L. G. (2007). Carotenoids and human health. Pharmacological Research, 55(3), 207–216.
Ratu, A. P., Silabi, N. F., & Citroreksoko, P. (2016). Uji antioksidan ekstrak pigmen karotenoid daan sitrulin pada kulit buah blewah (Curcumis melo L.) secara in vitro (metode DPPH). Jurnal Farmamedika (Pharmamedical Journal), 1(1), 1–11.
Shi, J., Le Maguer, M., Kakuda, Y., Liptay, A., & Niekamp, F. (1999). Degradation, lycopene dehydration, and isomerization in tomato. Food Research International, 32(1), 15–21.
Shui, G., Wong, S. P., & Leong, L. P. (2004). Characterization of antioxidant and change of antioxidant Levels during storage of Manalikara zapota L. Agricultural and Food Chemistry, 52(26), 7834–7841.
Simajuntak, P., Parwati, T., Lenny, L. E., Tamat, S. R., & Murwani, R. (2004). Isolasi dan identifikasi antioksidan dari ekstrak benalu teh (Scarrulaoortiana (korth) danser). Jurnal Ilmu Kefarmasian Indonesia, 2(1), 19–24.
Syaifuddin. (2015). Uji aktivitas antioksidan ekstrak daun bayam merah (Alternanthera amoena Voss) varietas mira segar dan rebus dengan metode DPPH. Skripsi Tidak Diterbitkan. Semarang: Universitas Islam Negeri Semarang.
Wall, M. M., Waddell, C. A., & Bosland, P. W. (2001). Variation in β-carotene and total carotenoid content in capsicum. Horticultural Science, 36(4), 746–749.
Warisno & Dahana, K. (2010). Peluang usaha dan budidaya cabai. Jakarta: PT Gramedia Pustaka Utama.
Winarno, F. G. (1989). Kimia pangan dan gizi. Jakarta: PT Gramedia.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Febryna V. Maddo, Siti Aminah , Afadil Afadil
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
