Antioxidant Activity Tests of Extract of Principle Crafts (Phaleria macrocarpa)
DOI:
https://doi.org/10.22487/j24775185.2019.v8.i3.pp125-129Keywords:
Antioxidant, phaleria macrocarpa, 2,2,-dyphenyl-1-picrilhidrazil (DPPH)Abstract
Phaleria macrocarpa contains secondary metabolite compounds such as alkaloids, terpenoids, saponins, polyphenols, tannins, sterols, and coumarins. Those metabolites are antioxidants and can be efficacious in the healing of various degenerative diseases such as cervical cancer and diabetes. This plant is widely grown in Tindaki Village, Parigi Mautong, Central Sulawesi. The aim of this study was to determine the antioxidant power of those phaleria macrocarpa fruit extract. This study was conducted by using maceration extraction technique with ethanol as solvent, and compound 2,2-difenil-1-picrilhidrazil (DPPH) as source of free radical, and vitamin C as the positive control. The UV-Vis spectrophotometer was used to measure the absorbance of the extract. Various concentrations of the extract used were 5 ppm, 15 ppm, 25 ppm, and 35 ppm. The results showed that the IC50 value of the fruit extract was 28.242 ppm, while the IC50 value of vitamin C was 19.302 ppm. These IC50 values show that vitamin C was a stronger antioxidant activity than the fruit extract, but the fruit extract was still as a very strong natural antioxidant category.
References
Agoes, A. (2010). Tanaman obat indonesia. Jakarta: Selemba Medika.
Apriyanti, M. (2012). 10 Tanaman obat paling berkhasiat dan paling dicari. Jakarta: Pustaka Baru Press.
Arini, S., Nurmawan, D., Alfiani ,F. & Hertiani, T. (2003). Daya antioksidan dan kadar flavonoid hasil ekstraksi etanol-air daging buah mahkota dewa (phaleria macrocarpa (Scheff.) Boerl.). Jurnal Buletin Penalaran Mahasiswa UGM, 10(1), 2-6.
Bahriul, P., Rahman, N. & Diah, A. W. M. (2014). Uji aktivitas antioksidan ekstrak daun salam (syzygium polyanthum dengan menggunanakan 1,1-difenil-2-pikrilhidrazil. Jurnal Akademika Kimia, 3(3), 143-149.
Gotawa, I. B. I., Sugiarto, S., Nurhadi, M., Widiyastuti, Y. Wahyono, S., & Prapti, I. J. (1999). Inventaris tanaman obat indonesia jilid V. Jakarta: Departemen Kesehatan. Badan Penelitian dan Pengembangan Kesehatan.
Harmanto, N. (2003). Conquering disease in unison with mahkota dewa. phaleria macrocarpa. Jakarta: First editon P.T. Mahkotadewa Indonesia.
Harmanto, N. (2002). Sehat dengan ramuan tradisional mahkota dewa. cetakan keempat. Tangerang: PT. Agromedia Pustaka.
Hermansyah, A. H. & Zhara, T. A. (2005). Skrining fitokimia dan uji aktivitas antioksidan ekstrak kulit batang laban (Vitex pubescens vahl). Jurnal JKK, 4(2), 67-71.
Harbome, J. B. (1987). Metode fitokimia penentuan cara modern menganalisis tumbuhan. Bandung: ITB.
Isnindar, S. W. & Erna, P. S. (2011). Isolasi dan identifikasi senyawa antioksidan daun kesemek (dipspyros kaki thunb) dengan metode DPPH (2,2-difenil-1-1 pikrilhidrazil). Jurnal Obat Tradisional,16(3),157-164.
Lu, Y. & Foo, L.Y. (2000). Antioxidant and radical scavenging activities of polyphenols from apple omace. Jurnal Food Chemistry, 68(1), 81-85.
Molyneux, P. (2003). The use of the stable free radical diphenylpicrylhydrazyl (dpph) for estimating antioxidant activity. Journal of Science and Technology, 26(2), 211-219.
Nur, K., Diah, A. W. M. & Nuryanti, S. (2016). Uji aktivitas ektrak daun palado (Agave angustifolia) sebagai antioksidan. Jurnal Akademika Kimia, 5(2), 73-78.
Parwati, N. K. F., Napitupulu, M. & Diah, A. W. M. (2014). Uji aktivitas antioksidan ekstrak daun binahong (Anredera cordifolia (tenore) steenis) dengan 1,1-difeni l-2-pikrilhidrazil (DPPH) menggunakan spektrofotometer uv-vi. Jurnal Akademika Kimia, 3(4), 206-213.
Ratna, D., & Tria, A. (2009). Penentuan kandungan flavonoid dari ekstrak metanol daging buah mahkota dewa (Phaleria macrocarpa scheff boerl). Jurnal Ilmu Kefarmasian Indonesia, 7(2), 65-71.
Soeksmanto, A. (2006). Pemberian ekstrak butanol Buah tua mahkota dewa (Phaleria macrocarpa) terhadap jaringan ginjal mencit (Mususculus). Jurnal BIODIVERSITAS, 7(3), 278-281.
Tonahi, J. M. M., Nuryanti, S. & Suherman (2014). Antioksidan dari daun sirih (Piper crocatum). Jurnal Akademika Kimia, 3(3), 158-164.
Winarno, H. & Katrin, E. (2008). Aktivitas sitotoksik fraksi-fraksi ekstrak etil asetat kulit batang mahkota dewa (Phaleria macrocarpa (Scheff.) Boerl) terhadap sel kanker manusia Jurnal Obat Tradisional, 13(45), 120-127.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2019 Author
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
