Antioxidant Activity Test of Muntingia calabura L. Fruit Extract using 1,1-diphenyl-2-picrylhydrazyl
DOI:
https://doi.org/10.22487/me.v17i2.1108Keywords:
Antioxidant, extract, ethanol, UV-VIS spectrophotometer, IC50, Muntingia calabura L., fruit, DPPHAbstract
Kersen, also known as Muntingia calabura L. is a fruit with numerous health benefits that can be ingested instead of medication. The goal of this study was to see how effective the extract Muntingia calabura L. was as an antioxidant. This study used DPPH (2,2-diphenyl-1-picrylhydrazyl) as a source of free radicals and vitamin C as a positive control to measure antioxidant activity. A UV-VIS spectrophotometer was used to determine the antioxidant activity of Muntingia calabura L. extract. The fruit powder of Muntingia calabura L. was extracted using the maceration process using ethanol as a solvent. The antioxidant activity of the extract was next examined at various concnetration of 20, 40, 60, and 80 ppm. Muntingia calabura L. extract had a strong antioxidant power with an IC50 value of 69.662 ppm, while vitamin C had also a strong antioxidant power with an IC50 value of 79.615 ppm, according to the findings. Muntingia calabura L. fruit extract is a good source of natural antioxidants, according to the IC50 value, because it has a high antioxidant activity value.
References
Dwi & Istikhomah, “Pengujian Aktivitas Ekstrak Daun Kersen (Muntingia calabura L.)”,Ujung Pandang: Balai Penelitian Kimia, 2010.
F. Yunahara, S. Setyorini, dan L. S. Witha, “Uji Aktivitas Antioksidan pada Buah Talok dengan Metode DPPH dan Rancimat dalam Seminar PATPI”, Fakultas Farmasi Universitas Pancasila, Jakarta, 2009, pp. 9-16.
D. K. Binawati, and S. Amilah, “Effect of Cherry Leaf (Muntingia Calabura L.) Bioinsecticides Extract Towards Mortality of Worm Soil (Agrotis Ipsilon) and Armyworm (Spodoptera Exiqua) on Plant Leek (Allium Fistolum),” Jurnal Wahana 61 (2), 2013, pp. 51–57 .
S. W. Isnindar, dan P. S. Erna, “Isolasi dan Identifikasi Senyawa Antioksidan Daun Kesemek (Dipspyros kaki Thunb.) Dengan Metode DPPH (2,2-difenil-1-1-pikrilhidrazil),” Majalah Obat Tradisional. Vol. 16(3), 2011, pp. 157-164.
A. B. Utomo, A. Suprijono, & A. Risdianto, “Uji aktivitas antioksidan kombinasi ekstrak sarang semut (Myrmecodia pendans) dan ekstrak teh hitam (Camellia sinensis O.K.var.assamica (mast.)) dengan metode DPPH (1,1-difenil-2-pikrilhidrazil),” 2008.
W. Selawa, M. R. J. Runtuwene, & G. Citraningtyas, ”Kandungan flavonoid dan kapasitas antioksidan total ekstrak etanol daun binahong [anredera cordifolia(ten.)steenis.],” Jurnal Ilmiah Farmasi, 2(1), 2013, pp.18-22.
T. Sunarni, S. Pramono, & R. Asmah, “Flavonoid antioksidan penangkap radikal dari daun kepel (stelechocarpus burahol (bl.) hook f. & th.),” Majalah Farmasi Indonesia, 18(3), 2007, pp. 111-116
P. Molyneux, ”The use of the stable free radical Diphenylpicryl-hydrazyl (DPPH) for estimating antioxidant activity,” Songklanakarin Journal of Science and Technology, 26(2), 2004, pp. 211-219.
Rizkayanti, A. W. M. Diah, & M. R. Jura, “Uji aktivitas antioksidan ekstrak air dan ekstrak etanol daun kelor (Moringa oleifera LAM),” Jurnal Akademika Kimia, 6(2), 2017, pp. 125-131.
S. Sulaeha, M. R. Jura, & N. Rahman, “Uji aktivitas antioksidan ekstrak etanol biji buah merah (Pandanus conoideus de vriese) asal Kabupaten Poso Sulawesi Tengah,” Jurnal Akademika Kimia, 6(3), 2017, pp. 170-174.
N. Sharon, S. Anam, & Yuliet, “Formulasi krim antioksidan ektrak etanol bawang hutan (Eleutherine palmifolia L.Merr),” Natural Science: Journal of Science and Technology, 2(3), 2013, pp. 111-122.
C. F. Zuhra, J. B. Tarigan, & H. Sihotang, “Aktivitas antioksidan senyawa flavonoid dari daun Katuk (Sauropus androgunus(L) Merr.)” Jurnal Biologi Sumatera, 3(1), 2008, pp. 7-10.
S. M. Khopkar, “Konsep dasar kimia analitik,”. Jakarta: UI-Press, 2014.
L. Lumempow, E. Suryanto, dan J. Paendong, “Ekstraksi Antioksidan dari Kulit Manggis (Garcinia mangostana L.)”. Research Report Engineering Science . 2, 2012, pp. 1-52
J. B. Harborne, “Metode fitokimia penentuan cara modern menganalisis tumbuhan”, Bandung: ITB, 1987.
S. Talapessy, E. Suryanto, & A. Yudistira, “Uji aktivitas antioksidan dari ampas hasil pengolahan sagu (Metroxylon sagu Rottb),” Pharmacon Jurnal Ilmiah Farmasi, 2(3), 2013, pp. 112-117
N. Larasati, “Studi aktivitas antioksidan dan ekstrak fisiko kimia tauco yang beredar di kota Malang,” Jawa Timur. Jurnal Pangan dan Agroindustri, 5(2), 2017, pp. 85-95
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Zela, Anang Wahid Muhammad Diah
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors who publish with Jurnal Media Eksakta agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY-SA 4.0) that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
